Keywords: Gold Nanoprism; Green Synthesis; Penicillium citrinum; Transmission , triangular gold nano plates using algal solutions of. , S. S. Shankar, A. Rai, B. Ankamwar, A. Singh, A. Ahmad and M. Sastry, “ Biological Synthesis of Triangular Gold Nanoprisms,” Nature Materials, Vol. 3, No . Synthesis of triangular gold nanoprism using lemon grass leaf extract showed UV-Visible spectra of gold nanoparticles by bio-reduction of Chloroauric acid in.
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Due to symmetric face nanopeisms cubic fcc biooogical of gold nanoparticles, the formation of anisotropic structures like cube, prism, star, branched nanoparticles require a selective capping agent during growth The synthesis of nanoparticles using biological approaches offers several advantages than chemical synthesis like environmentally acceptable solvent, eco friendly reducing and capping agents.
This microbial synthesis of GNPs could be explained by the fact that microorganisms often face metal ions or metals in their environment and thereby developed mechanism to detoxify metals by changing the redox states of metal ions and or intracellular precipitation of metals forming a basis for synthesis of nanoparticles .
It was quite exciting for us to find this bioprocess oc mycelial biomass suspended in double distilled water in presence of chloroauric acid could produce GNPs on their surfaces.
This report provides for the first time rapid novel environment friendly cell surface based synthesis of stable gold nanoprisms at room temperature using Penicillium citrinum MTCC biomass.
UV-Visible spectra of gold nanoparticles by bio-reduction of Chloroauric acid in aqueous solution was recorded in Jasco UV-Visible spectrophotometer V operated at a resolution of 1 nm in absorption mode. Synthesis of spherical gold nanoparticles GNPs have been reported using the fungus Synthesiss oxysporum and Verticillium sp.
The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline trixngular nature .
In recent years the synthesis of stable gold nanoparticles is one of the most nqnoprisms areas of research in the field of nanotechnology because of their applications in a number of areas like bio-imaging, bio-medicine, bio-sensors, bio-labels, catalysis and others . The physical nature of GNPs indicated that most of the nanoparticles were prism shaped.
Biological Synthesis of Colloidal Gold Nanoprisms Using Penicillium citrinum MTCC
FTIR analysis also showed that protein molecules were involved in stabilization. FTIR spectra of gold nanoparticles.
Although some rod shaped, triangular, cubical, very small circular 3 – 4 nm diameter and rectangular GNPs were present in smaller amounts Figures 3 A and B. The purple colour of Penicillium citrinum biomass is due to the interaction of GNPs with light . However, there is limited information available regarding the synthesis of GNPs using fungus. The biomolecules present in the cell surface of Penicillum citrinum may perform three important functions: To investigate the physical nature of gold nanoparticles transmission electron microscopic analysis was done.
By conventional chemical and physical methods nanocubes , nanorods , nanowires , nanoplates  can be synthesized but that require high environmental cost. Each experiment was repeated thrice using freshly grown culture of P.
Typically 2 g biomass fresh weight was dispersed in 20 ml of double distilled water pH 5. In all these respects biological approach would become a better choice for the synthesis of gold nanoparticles GNPs. Received January 16 th; revised February 23 rd; accepted April 5 th The authors would like to thank Shantiswarup Sinha and Prof.
The d-spacings calculated from FFT images of a triangular gold nanoparticle and a gold nanoprism were 2.
Biological synthesis of triangular gold nanoprisms.
Thus this is quite exciting for us to synthesize gold nanoprism for first time using biological good. However, bio application of large nano particles greater than 50 nm are limited due to reduced endocytosis and poor renal clearance . Development of strain in crystals of gold nanoprism was observed from the analysis of crystals. We emphasized on bio-inspired synthesis of gold nanoparticles by Penicillium citrinum MTCC in room temperature.
The zeta potential of GNPs was measured to know their colloidal stability and the triangulad of the charge carried in their surface. The Scherrer ring patterns indicate the fcc gold which is nanocrystalline in nature.
B A selected area electron diffraction pattern of GNPs. The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature Biomaterials have inspired a growing research effort in which biomolecules are used to synthesize and assemble materials in the laboratory.
The strain was subcultured on potato dextrose agar PDA. Synthesis of triangular gold nanoprism using lemon grass leaf extract showed large size of nanoprisms ranging from – nm length and truncated vertices .
Biological synthesis of triangular gold nanoprisms.
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The process was repeated for three times and finally the centrifuged part containing GNPs were redispersed in double distilled water and subjected to FTIR spectroscopy. There are other reports which showed the intracellular synthesis of spherical GNPs in live Alfaalfa plants , truncated triangular and spherical GNPs from sun dried Cinamonum camphora leaf extract , triangular gold nanoprism from lemon grass extract , hexagonal, prism and spherical shaped GNPs using plant surfactants , triangular gold nano plates using algal solutions of Chlorella vulgaris  and Sargassum sp.
All chemical reagents were purchased from Sigma St. The UV-Visible spectral scan of dispersed gold nanoparticles GNPs solution triangualr absorption maxima at nm due to surface plasma resonance SPR of gold nanoparticles. In addition gold nanoprism offers several advantages nanoprisme biomedical applications.
Therefore a growing demand has been accumulated to develop environment friendly approaches to synthesize nanomaterials Green Chemistry. Then the biomass was harvested by sieving through a plastic filter and washed several times with double distilled water to remove any traces of media components.
The GNPs then resuspended in double syynthesis water and again centrifuged. These systems are resourceful and assemble a vast assortment of materials using precursors that are readily available and abundant in the biosphere. Free amine, carboxyl or phosphate groups present in the protein can interact with gold nanoparticles [32,37] and possibly involved in stabilization of GNPs.
FFT image also showed an elongation of the diffraction reflexes. Moreover, the synthesis of other anisotropic nanostrctures of gold like nano rod, cube, triangle which are present in smaller quantities as per TEM images may be achieved by further tuning the synthesis protocol using the fungus. For FTIR spectrum analysis the GNPs were centrifuged at 10, rpm for 10 minutes to remove free proteins or other compounds present in the solution.
Metal nanoparticles are produced mainly by means of chemical processes involving chemical aggressive reducing agents e.
Some rod shaped, triangular, cubical, very small circular 3 – 4 nm diameter and rectangular GNPs were present in smaller amounts Figures A and B. In our study, GNPs formed a single population in the size range of 10 – 40 nm. GNPs triangu,ar was found to be in the range of 10 – 40 nm with maximum population centered on 20 nm Figure 2.